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Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]
Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] from Abcam
Be the first to write a review!
Citations:
(64)
Supplier Page
Supplier Page from
Abcam for
Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab22506
Antibody Type
Primary
Clonality
Monoclonal
Quantity
10 UG, 100 UG
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections)
Clone
MAC387
Isotype
IgG1
Host
Mouse, Nude mouse
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Reactivity
Human, Mouse
Target
S100A9 + Calprotectin (S100A8/A9 complex)
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (64)
(1)
Urinary single-cell sequence analysis of the urinary macrophage in different outcomes of membranous nephropathy
Clinical Kidney Journal
November 30, 2023
Xi Liu, Yu Zhao, Yangyang Niu, Qionghong Xie, Hao Nie, Yun Jin, Yingying Zhang, Yuqiu Lu, Saiya Zhu, Wei Zuo,
et al.
(Shanghai) Company Ltd], CD68 [GM081402,
1:200
, Gene Tech (Shanghai) Company Ltd] and S100A8/A9 complex (
ab22506
,
1:1000
,
Abcam
). The positive cells for CD3, CD20, CD68 and S100A8/A9
staining
were counted under a
(2)
The Cxcr2+ subset of the S100a8+ gastric granylocytic myeloid-derived suppressor cell population (G-MDSC) regulates gastric pathology
Frontiers in Immunology
September 8, 2023
Krystal D. Kao, Helmut Grasberger, Mohamad El-Zaatari
performed as described previously (10), using antibodies against the following targets: calprotectin (#
ab22506
,
Abcam
);
CD11b
-AF594 (
clone
M1/70, #
101254
,
BioLegend
); Cxcr2-AF488 (#
FAB2164G
,
R&D Systems
); GSII-FITC
(3)
Molecular Magnetic Resonance Imaging of Aneurysmal Inflammation Using a Redox Active Iron Complex
Investigative Radiology
September 1, 2023
Robert M. King, Matthew J. Gounis, Eric J. Schmidt, Anita Leporati, Eric M. Gale, Alexei A. Bogdanov
serum in TBS, pH 7.5 for 30 min.
For IHC
detection of calprotectin anti-(S100A8/A9 complex)/S100A9 (
ab22506
Abcam
, Waltham MA) antibody was covalently labeled by using
digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester
(4)
Organic Matrix Derived from Host–Microbe Interplay Contributes to Pathological Renal Biomineralization
ACS Nanoscience Au
August 16, 2023
Yushi Bai, Yongmei Wang, Misun Kang, Claire M. Gabe, Sudarshan Srirangapatanam, Austin Edwards, Marshall Stoller, Stefan J. Green, Shaul Aloni, Nobumichi Tamura,
et al.
anti-myeloperoxidase (rabbit monoclonal,
ab208670
,
Abcam
, Cambridge), and anti-calprotectin (
mouse
monoclonal,
ab22506
,
Abcam
, Cambridge) were incubated on the sections under static conditions overnight at 4 °C. The sections
(5)
Phosphorylcholine and KR12-Containing Corneal Implants in HSV-1-Infected Rabbit Corneas
Pharmaceutics
June 5, 2023
Kamal Malhotra, Oleksiy Buznyk, Mohammad Mirazul Islam, Elle Edin, Sankar Basu, Marc Groleau, Delali Shana Dégué, Per Fagerholm, Adrien Fois, Sylvie Lesage,
et al.
UK",
1:50
lymphatic vessels,"Anti-Lyve 1,
ab14917
,
Abcam
, Cambridge, UK",
1:100
Macrophages
,"Anti-MAC 387,
ab22506
,
Abcam
, Cambridge, UK",
1:100
Nerves,"
neuron
-specific
Anti-beta III Tubulin
[Tu-20],
ab7751
,
Abcam
,
(6)
Fibroblast Growth Factor 2 Is Produced By Renal Tubular Cells to Act as a Paracrine Factor in Maladaptive Kidney Repair After Cisplatin Nephrotoxicity
Laboratory investigation; a journal of technical methods and pathology
March 1, 2023
Hu X, Ma Z, Li S, Wen L, Huo Y, Wu G, Manicassamy S, Dong Z.
(
SK-5105
;
Vector Laboratories
). The primary antibodies for immunostaining included antimacrophage (
ab22506
,
Abcam
) and antie-α-SMA (
ab5694
, Abcam). Sirius red
staining
was performed according to a standard protocol
(7)
Comparative lipid profiling of murine and human atherosclerotic plaques using high-resolution MALDI MSI
Pflügers Archiv - European Journal of Physiology
February 1, 2022
Pegah Khamehgir-Silz, Stefanie Gerbig, Nadine Volk, Sabine Schulz, Bernhard Spengler, Markus Hecker, Andreas H. Wagner
stained according to standard
procedures
for MAC387 immunopositive infiltrating
macrophages
(
1:100
,
ab22506
,
Abcam
, UK) and
CD31
(
1:100
, M0823, Agilent Dako, USA) using
donkey
-anti-
mouse
cy3 [715-166-020] and
(8)
Dexamethasone modulates immature neutrophils and interferon programming in severe COVID-19
Nature Medicine
January 1, 2022
Sarthak Sinha et al.
with 10% normal
donkey
serum in PBS (with 0.5% Triton X-100), and primary antibodies (S100A8/9,
Abcam
,
ab22506
;
IFITM1
,
Abcam
,
ab233545
) were incubated at 4 °C overnight, followed by
incubation
with
donkey
anti-rabbit
(9)
Chemokine‐Capturing Wound Contact Layer Rescues Dermal Healing
Advanced Science
September 1, 2021
Lucas Schirmer, Passant Atallah, Uwe Freudenberg, Carsten Werner
followed by primary antibody
incubation
in TBST (Sigma Aldrich) for 2 h. Mouse anti‐S100A9 (
1:100
,
Abcam
,
ab22506
) was used to evaluate inflammatory
neutrophil
and
macrophage
presence. After washing three times in
(10)
Transcriptional profiling of pediatric cholestatic livers identifies three distinct macrophage populations
PLOS ONE
January 7, 2021
Sarah A. Taylor et al.
were stained for CD68 (
Abcam
ab955
),
CD69
(
Abcam
ab233396
),
C1Q
(
Abcam
ab268120
), and S100A8/9 (
Abcam
ab22506
). Whole slide fluorescent imaging was performed followed by multispectral imaging of three 2.01 mm x
(11)
Interleukin-17A pathway target genes are upregulated in Equus caballus supporting limb laminitis
PLOS ONE
December 10, 2020
Lynne Cassimeris, Julie B. Engiles, Hannah Galantino-Homer
sections.
Immunofluorescence
Tissue sections were also used to localize calprotectin using
mouse
antibody
ab22506
(
1:5000
;
Abcam
) recognizing S100A9, followed by goat anti mouse IgG-Alexa 488 (
1:100
), based on a protocol
(12)
Prognostic Role of Survivin and Macrophage Infiltration Quantified on Protein and mRNA Level in Molecular Subtypes Determined by RT-qPCR of KRT5, KRT20, and ERBB2 in Muscle-Invasive Bladder Cancer Treated by Adjuvant Chemotherapy
International Journal of Molecular Sciences
October 8, 2020
Thorsten H. Ecke et al.
(concentration
1:5
;
ab845
,
Abcam
, U.K.) and mouse monoclonal IgG1 antiMAC387 (concentration
1:500
;
ab22506
,
Abcam
, U.K.), which detects the myelomonocytic L1 molecule calprotectin. CLEVER-1 (common lymphatic
(13)
Iron imaging in myocardial infarction reperfusion injury
Nature communications
June 29, 2020
Moon BF, Iyer SK, Hwuang E, Solomon MP, Hall AT, Kumar R, Josselyn NJ, Higbee-Dempsey EM, Tsourkas A, Imai A,
et al
.
LS‑C755633
,
1:100
dilution), Cathepsin-S antibody (
GeneTex
:
GTX114350
) and a MAC-387 antibody (
Abcam
:
ab22506
), respectively. Tissue
fiducial markers
, RV insertion, papillary
muscle
shape, length of the LV, and
(14)
A Metabolic Intravascular Platform to Study FDG Uptake in Vascular Injury
Cardiovascular Engineering and Technology
June 1, 2020
F. Franchi, M. Olthoff, J. Krier, C. Noble, M. Al-Hijji, V. Ramaswamy, T. Witt, M. Burke, M. Benscoter, A. Lerman,
et al.
Additional unstained slides were used
for immunohistochemistry
staining
for MAC387 (
macrophage
marker,
Abcam
ab22506
,
1:50
overnight at 4°C), VCAM-1 (vascular cell adhesion molecule-1,
Abcam
ab98954
, 8 μg/ml overnight
(15)
Obeticholic Acid Ameliorates Valproic Acid–Induced Hepatic Steatosis and Oxidative Stress
Molecular Pharmacology
May 1, 2020
Zhibo Gai, Evelin Krajnc, Sophia L. Samodelov, Michele Visentin, Gerd A. Kullak-Ublick
microwave-based antigen-retrieval
technique
. The antibodies used in this study were against the
macrophage
marker MAC387 (
ab22506
;
Abcam
), 4hydroxynonenal (4-HNE) (
ab46545
;
Abcam
), and the
endoplasmic reticulum
chaperone protein Grp78
(16)
Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology
Nature protocols
May 1, 2020
Bucur O, Fu F, Calderon M, Mylvaganam GH, Ly NL, Day J, Watkin S, Walker BD, Boyden ES, Zhao Y.
,
Agilent Technologies
,
M085129-2
,AB_2811108
IgD
,
Goat
,poly,
SouthernBiotech
,
2030-01
,AB_2795623 S100A8,Mouse,mono,
Abcam
,
ab22506
,AB_447111
MCT1
,
Chicken
,poly,
Sigma Aldrich
,
AB1286-I
,AB_11212410
Tom20
,Rabbit,poly,
Sigma Aldrich
,
HPA011562
,AB_1080326 Nephrin,Rabbit,poly,Santa
(17)
Mfsd2a and Spns2 are essential for sphingosine-1-phosphate transport in the formation and maintenance of the blood-brain barrier
Science advances
May 1, 2020
Wang Z, Zheng Y, Wang F, Zhong J, Zhao T, Xie Q, Zhu T, Ma F, Tang Q, Zhou B,
et al
.
PA5-28584
,
Invitrogen
), S100A8 (rabbit,
1:100
;
ab92331
,
Abcam
), S100A9/calprotectin (
mouse
,
1:200
;
ab22506
,
Abcam
), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (rabbit,
1:1000
;
ab181602
,
Abcam
), Mfsd2a
(18)
Effects of Farnesiferol B on Ischemia-Reperfusion-Induced Renal Damage, Inflammation, and NF-κB Signaling
International Journal of Molecular Sciences
December 12, 2019
Lu Zhang, Xianjun Fu, Ting Gui, Tianqi Wang, Zhenguo Wang, Gerd A. Kullak-Ublick, Zhibo Gai
lipocalin (
NGAL
,
ab63929
,
Abcam
), Ly-6B (NBP2-13077), NFκB
p65
(sc-372, Santa Cruz, CA, USA), and MAC387 (
ab22506
,
Abcam
). Sections were treated with the Envision+ DAB kit (Dako, Basel, Switzerland) according to the
(19)
Immunohistochemical characterisation of the hepatic stem cell niche in feline hepatic lipidosis: a preliminary morphological study
Journal of Feline Medicine and Surgery
February 1, 2019
Chiara Valtolina, Joris H Robben, Robert P Favier, Jan Rothuizen, Guy CM Grinwis, Baukje A Schotanus, Louis C Penning
code,Dilution K19,
Mouse,IgG1
,b170,Prot K,O/N 4°C,
Novocastra
,
NCL
-
CK19
,
1:100
MAC387,
Mouse,IgG1
,MAC387,Prot K,O/N 4°C,
Abcam
,
ab22506
,
1:1000
α-SMA,
Mouse,IgG2a
,1A4,None,60 mins RT,
BioGenex
,
MU128-UC
,
1:200
LAM,Rabbit,Ig,,Prot K,O/N 4°C,
Abcam
,
ab11575
,
1:100
Ki67,
Mouse,IgG1
,
MIB1
,Citrate
(20)
High-resolution Imaging of Myeloperoxidase Activity Sensors in Human Cerebrovascular Disease
Scientific Reports
December 1, 2018
Youssef Z. Wadghiri, Dung Minh Hoang, Anita Leporati, Matthew J. Gounis, Aurora Rodríguez-Rodríguez, Mary L. Mazzanti, John P. Weaver, Ajay K. Wakhloo, Peter Caravan, Alexei A. Bogdanov
granulocytes
,
monocytes
and
macrophages
) were detected by using anti-calprotectin (S100A8/A9 complex) antibody (
ab22506
,
Abcam
); ND- not done.
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Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] from Abcam
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
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